Methods Development

A major interest of the lab is the development and optimisation of methods for high-resolution cryo-EM. This has led us to investigate

• Refinement of 3D reconstructions using single-particle images [1] [2] [3] [4]
• Particle selection from micrographs [5]
• Resolution measurement [6] [7]
• High-resolution 3D reconstruction of helical specimens and filaments [8] [9]
• CTF estimation from electron micrographs [10] [11]
• DQE measurement of electron detectors [12]
• Beam-induced motion during cryo-EM data collection [13]
• Movie-collection and processing to enhance resolution in cryo-EM [14] [15]
• Magnification distortion correction in electron micrographs [16]
• Likelihood-based classification of cryo-EM images [17] [18]
• Single-protein detection in crowded molecular environments in cryo-EM images [19]
• Fast and user-friendly single-particle image processing [20]
• 2D template matching [21] [22] [23] [24]

References