Quantitative characterization of electron detectors for transmission electron microscopy
Publication Type
Journal Article
Year of Publication
2013
Refereed Designation
Refereed
Journal
J Struct Biol
Volume
184
Pagination
385-393
Date Published
2013 Nov 1
ISSN
1095-8657
Citation Key
4236
Abstract
A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of their modulation transfer function (MTF) and detective quantum efficiency (DQE). However, the performance of these new detectors needs to be carefully monitored in order to optimize imaging conditions and check for degradation over time. We have developed an easy-to-use software tool, FindDQE, to measure MTF and DQE of electron detectors using images of a microscope's built-in beam stop. Using this software, we have determined the DQE curves of four direct electron detectors currently available: the Gatan K2 Summit, the FEI Falcon I and II, and the Direct Electron DE-12, under a variety of total dose and dose rate conditions. We have additionally measured the curves for the Gatan US4000 and TVIPS F416 scintillator-based cameras. We compare the results from our new method with published curves.
Alternate Journal
J. Struct. Biol.