Ewald sphere correction for single-particle electron microscopy

Publication Type

Journal Article

Year of Publication

2006

Journal

Ultramicroscopy

Volume

106

Pagination

376-82

Date Published

Mar

Accession Number

16384646

Abstract

Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction.

Keywords

Algorithms, Data Display, Fourier Analysis, Imaging, Three-Dimensional/*methods, Microscopy, Electron/*methods, Viruses/chemistry/ultrastructure

upload

Attachment Size
Wolf_Ultra2006.pdf 311.43 KB