FREALIGN: high-resolution refinement of single particle structures

Publication Type

Journal Article

Year of Publication

2007

Authors

Journal

J Struct Biol

Volume

157

Pagination

117-125

Abstract

The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page.

Keywords

Algorithms, Electron, Image Enhancement, Imaging, Microscopy, Particle Size, Software, Software Design, Three-Dimensional

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