Frealign: An exploratory tool for single-particle cryo-EM

Publication Type

Book Chapter

Year of Publication

2016

Authors

Volume

579

Pagination

191-226

ISBN

00766879

Abstract

Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possible resolution. It provides a number of refinement parameters and options that allow users to tune their refinement to achieve specific goals, such as masking to classify selected regions within a particle, control over the refinement of specific alignment parameters to accommodate various data collection schemes, refinement of pseudosymmetric particles, and generation of initial maps. This chapter provides a general overview of Frealign functions and a more detailed guide to using Frealign in typical scenarios.

Keywords

asymmetry, classification, contrast transfer function, high resolution, masking, refinement

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