Below is a stack (17 GB) of 4,339 images (MRC/CCP4 format) of rotavirus triple-layered particles (TLPs ) collected at 300 kV and a calibrated magnification of 56,770, giving a pixel size on the specimen of 1.233 Å. Alignment parameters determined by Frealign for each particle in the stack can also be found below, as well as a reconstruction of TLP (also MRC/CCP4 format). Ewald sphere correction was not performed.
To download the stack, please download all 9 parts below (TLP_stack_aa.part.gz - TLP_stack_ai.part.gz) and combine them to a single stack. On a Linux/UNIX system, you can use the attached script to download all parts and restore the stack. After downloading the script, type
chmod +x get_TLP_stack.b
If you download the partial stacks manually use this command to restore the stack:
gunzip TLP_stack_*.part.gz > TLP_stack.mrc
The file TLP_stack.mrc will contain the stack with all 4,339 images (the 9 partial stacks can be deleted).
Please download one file at a time or use the supplied scripts to download files sequentially. Attempts to download more than one file at a time will cause a Service Temporarily Unavailable message to be displayed.