NADH:Ubiquinone Oxidoreductase (Bovine Mitochondrial Complex I)
Attached below are two stacks (387 MB total) with a total of 6,184 images (MRC/CCP4 format) of NADH:ubiquinone oxidoreductase (complex I) . The 2914 images were recorded on a Philips CM12 microscope running at 120 keV and 3270 images were recorded on a Hitachi HF2000 electron microscope running at 200 keV. Both data sets were recorded under low-dose conditions, at a magnification of 60,000x, and with a defocus of ~3 µm and 3.7 - 6.7 µm, respectively. The pixel size of the final reconstruction is 4.667 Å. Alignment parameters determined by Frealign for each particle in the stack can also be found below, as well as a reconstruction of NADH (also MRC/CCP4 format; a B-factor of -800.0 Å2 and an 22.0 Å low-pass filter were applied using the program bfactor to sharpen the density) and a script to calculate a reconstruction using Frealign.