EF-G-ribosome pre-translocation complex

The attached archive contains Frealign parameter files and scripts that were used to calculate 15 classes from 1.3 million single particle images of an E. coli EF-F-ribosome preparation [1]. The data was collected at 300 kV on an FEI Titan Krios microscope and Falcon I direct electron detector at a calibrated magnification of 133,333, giving a pixel size on the specimen of 1.04 Å. The particle stack has 512 GB (in MRC/CCP4 format) and is available upon request as it is too large to be hosted on this web page.