TMV in Ice - Micrographs
Six images (MRC/CCP4 format) are available for download to test the Spider scripts used for the helical processing of Tobacco Mosaic Virus (TMV, Figure). They are images of TMV in ice at a calibrated magnification of 1.163 Å/pixel. These images were recorded on an FEI F30 at 200 kV with a dose of 15 e-/Å2 and an underfocus between 1.5 and 4.0 µm. Please see [1] for details.
Furthermore, a stack of TMV segments is available for download that was used to reconstruct TMV using Frealign v8.09, which implements a helical symmetry operator. The reconstruction and Frealign parameter files are also included for download below.
Please download one file at a time or use the supplied scripts to download files sequentially. Attempts to download more than one file at a time will cause a Service Temporarily Unavailable message to be displayed.
| Image | Calibrated mag | Scan pixel size | Beam voltage | Cs | Defocus parameters (CTFFIND3) |
|---|---|---|---|---|---|
| A00 | 60190 | 7.0 µm | 200 kV | 2 mm | 18098.07 Å, 15003.80 Å, 7.47 ° |
| A02 | 60190 | 7.0 µm | 200 kV | 2 mm | 16982.86 Å, 12757.80 Å, 14.38 ° |
| A06 | 60190 | 7.0 µm | 200 kV | 2 mm | 31700.83 Å, 27762.51 Å, 7.64 ° |
| A08 | 60190 | 7.0 µm | 200 kV | 2 mm | 32206.58 Å, 29185.52 Å, 3.06 ° |
| A12 | 60190 | 7.0 µm | 200 kV | 2 mm | 40817.04 Å, 36379.68 Å, 10.39 ° |
| A14 | 60190 | 7.0 µm | 200 kV | 2 mm | 39239.23 Å, 34608.70 Å, 16.16 ° |
