Six images (MRC/CCP4 format) are available for download to test the Spider scripts used for the helical processing of Tobacco Mosaic Virus (TMV, Figure). They are images of TMV in ice at a calibrated magnification of 1.163 Å/pixel. These images were recorded on an FEI F30 at 200 kV with a dose of 15 e-/Å2 and an underfocus between 1.5 and 4.0 µm. Please see  for details.
Furthermore, a stack of TMV segments is available for download that was used to reconstruct TMV using Frealign v8.09, which implements a helical symmetry operator. The reconstruction and Frealign parameter files are also included for download below.
Please download one file at a time or use the supplied scripts to download files sequentially. Attempts to download more than one file at a time will cause a Service Temporarily Unavailable message to be displayed.
|Image||Calibrated mag||Scan pixel size||Beam voltage||Cs||Defocus parameters (CTFFIND3)|
|A00||60190||7.0 µm||200 kV||2 mm||18098.07 Å, 15003.80 Å, 7.47 °|
|A02||60190||7.0 µm||200 kV||2 mm||16982.86 Å, 12757.80 Å, 14.38 °|
|A06||60190||7.0 µm||200 kV||2 mm||31700.83 Å, 27762.51 Å, 7.64 °|
|A08||60190||7.0 µm||200 kV||2 mm||32206.58 Å, 29185.52 Å, 3.06 °|
|A12||60190||7.0 µm||200 kV||2 mm||40817.04 Å, 36379.68 Å, 10.39 °|
|A14||60190||7.0 µm||200 kV||2 mm||39239.23 Å, 34608.70 Å, 16.16 °|