Below is a stack (731 MB) of 1,299 helical segments (Spider format) of Ndc80/microtubule complex () collected at 200 kV and a nominal magnification of 51,200, giving a pixel size on the specimen of 2.48 Å. Alignment parameters determined by Frealign for each segment in the stack can also be found below, as well as a reconstruction of the complex (also Spider format). Run the attached script to recalculate the reconstruction from the image stack and alignment parameters. The tar file can be unpacked using
tar xvzf ndc80_mreconstruct.tar.gz
An amplitude scaled version of the map is also available.
Please download one file at a time or use the supplied scripts to download files sequentially. Attempts to download more than one file at a time will cause a Service Temporarily Unavailable message to be displayed.