Synthetic 70S ribosome datasets

The attached dataset and parameter files were generated by Dmitry Lyumkis at The Scripps Research Institute for the purpose of evaluating the performance of the maximum-likelihood multi-reference refinement algorithm [1] implemented in Frealign. The image data is stored in MRC/CCP4 format using a pixel size of 2.52 Å, assuming a beam energy of 200 keV and a spherical aberration coefficient of the objective lens of 2.0 mm. The files can also be downloaded from the Scripps NRAMM web page.