EF-G-ribosome pre-translocation complex
A Frealign parameter file and the particle stack (in MRC/CCP4 format) that were used to calculate 15 classes from 1.3 million single particle images of an E. coli EF-F-ribosome preparation [1] can be downloaded from the EMPIAR database. The data was collected at 300 kV on an FEI Titan Krios microscope and Falcon I direct electron detector at a calibrated magnification of 133,333, giving a pixel size on the specimen of 1.04 Å.
