Reconstructions of Abeta(1-40) amyloid fibrils

Twelve fibril reconstructions

All maps below are stored in Spider format and were reconstruction from single fibrils, recorded on a Philips CM12 at an underfocus between 2.1 and 2.3 μm. The reconstructions were low-pass filtered with a cosine-edged mask to a resolution of 20 Å. This value exceeds the resolution indicated by the FSC = 0.5 criterion (see below) and, therefore, the reconstructions include some of the noisier signal at higher resolution. Please see [1] for details.

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Image Nominal mag Beam voltage Scan pixel size Pixel decimation Map pixel size Resolution (FSC = 0.5)
Fibril1 60000 120 kV 7.0 µm 4 x 4 4.67 Å 33 Å
Fibril2 60000 120 kV 7.0 µm 4 x 4 4.67 Å 34 Å
Fibril3 60000 120 kV 7.0 µm 4 x 4 4.67 Å 30 Å
Fibril4 60000 120 kV 7.0 µm 4 x 4 4.67 Å 33 Å
Fibril5 60000 120 kV 7.0 µm 4 x 4 4.67 Å 33 Å
Fibril6 60000 120 kV 7.0 µm 4 x 4 4.67 Å 36 Å
Fibril7 60000 120 kV 7.0 µm 4 x 4 4.67 Å 39 Å
Fibril8 60000 120 kV 7.0 µm 4 x 4 4.67 Å 33 Å
Fibril9 60000 120 kV 7.0 µm 4 x 4 4.67 Å 32 Å
Fibril10 60000 120 kV 7.0 µm 6 x 6 7.00 Å 30 Å
Fibril11 60000 120 kV 7.0 µm 4 x 4 4.67 Å 24 Å
Fibril12 60000 120 kV 7.0 µm 4 x 4 4.67 Å 26 Å

References