cisTEM

cisTEM is user-friendly software to process cryo-EM images of macromolecular complexes and obtain high-resolution 3D reconstructions from them. It was originally developed by Tim Grant, Alexis Rohou and Nikolaus Grigorieff and comprises a number of tools to process image data including movies, micrographs and stacks of single-particle images, implementing a complete “pipeline” of processing steps to obtain high-resolution single-particle reconstructions. cisTEM is distributed under the Janelia Farm Research Campus Software Copyright 1.1 and can be downloaded here and below. We recommend downloading and using the pre-compiled binaries, rather than compiling the source code, for best performance. New users are encouraged to follow the tutorial, which provides a quick way to become familiar with the most important functions of cisTEM. [1] [2] [3] [4] [5] [6] [7] [8] [9]

Please visit cistem.org for help.

The development of cisTEM began in 2015 at HHMI's Janelia Research Campus in Ashburn, Virginia. Contributions to the program code were made by:

• Tim Grant
• Alexis Rohou
• Nikolaus Grigorieff
• Benjamin Himes
• Johannes Elferich
• Kexin Zhang
• Lingli Kong

Support

Please visit the cistem.org documentation pages for help. If you encounter any problems using cisTEM , or have questions, please use the cisTEM forum.

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References