Effect of mol_mass parameter

Submitted by rdrighetto on Tue, 2015-09-22 05:05

Forums

Frealign

Hi,

I am wondering what exactly is the role of the mol_mass parameter in the refinement? Is it employed in the definition of the particle Wiener filter [1]? Or is it just used to compute the final map threshold?

Thanks,

Ricardo

References


Jianhao Thu, 2015-12-24 04:19

I would wonder if I have a particle of about 20~25nm in diameter, how to calculate a proper value for mol_mass parameter?

Best regards!
Jianhao

niko Thu, 2015-12-24 08:29

The molecular mass should be known from your biochemical preparation of the protein. If not, you can estimate it from the volume by using a value of 0.81 Da/Å^3. If you do not know the volume either just use the best estimate you have and update it if you get to higher resolution and can estimate the mass better.