Effect of mol_mass parameter
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Hi,
I am wondering what exactly is the role of the mol_mass parameter in the refinement? Is it employed in the definition of the particle Wiener filter [1]? Or is it just used to compute the final map threshold?
Thanks,
Ricardo
Yes, the mol mass is used to
Yes, the mol mass is used to determine the Wiener filter constant.
I would wonder if I have a
I would wonder if I have a particle of about 20~25nm in diameter, how to calculate a proper value for mol_mass parameter?
Best regards!
Jianhao
The molecular mass should be
In reply to I would wonder if I have a by Jianhao
The molecular mass should be known from your biochemical preparation of the protein. If not, you can estimate it from the volume by using a value of 0.81 Da/Å^3. If you do not know the volume either just use the best estimate you have and update it if you get to higher resolution and can estimate the mass better.