Gun Tilt Refinement
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Hi, I have a question about gun tilt refinement in FREALIGN.
I tried different values for TX and TY (even at 0.2 mrad instead of 0.02), but always got exactly the same resolution number and FSC curve.
In mult_hreconstruct_n.com file, I put in:
1.,${dstep},60.0,${thresh},${cs},${kV},0.2,0.2 !RELMAG,DSTEP,TARGET,THRESH,CS,AKV,TX,TY
How can I be sure the program actually reads in the TX and TY values I specified?
Thanks!
Rui
The TX, TY values should be
The TX, TY values should be printed as part of the header section of the output parameter file. Did the output maps also look exactly the same for different values?
Hi, Niko, The output files
In reply to The TX, TY values should be by niko
Hi, Niko,
The output files such as 13pf_2_r1_mult_reconstruct.log and 13pf_2_r1.res contain the correct TX, TY values I put in, but the output maps look close to identical.
I only tried different TX TY values for the reconstruction step, but using the same parameter file from refinement.
Could you try something much
In reply to Hi, Niko, The output files by Rui Zhang
Could you try something much larger, like 2 or 20, to see if this has an effect?
OK. I will try that. Thanks!
In reply to Could you try something much by niko
OK. I will try that. Thanks!
Hi, by setting TX and TY to 2
In reply to OK. I will try that. Thanks! by Rui Zhang
Hi, by setting TX and TY to 2 mrad, the reconstruction did get significantly worse.
I'll try more values by grid search to see if I can improve the structure. Thanks!