I'm trying to use Signature to select the particles from my micrograph, given a template.
I would like to get coordinates the picked particle and angle respect to the template to calculate nearest neighbor distance and angle distribution. After importing, I find that both micrograph and template are rotated by counterclockwise 90 degree and mirror-imaged across the mirror plane lying along y = x of the rotated image.
I guess that this manipulation during the import might not affect the distance distribution; I'm not certain of how it affect the angle distribution, compared if doing the same thing with un-rotated and un-mirror imaged micrograph and template.
Can anyone suggest the way to counter this problem? Is it possible to adjust the program such that it doesn't do such things for the import?