frealign_calc_variance

Submitted by lukater on Thu, 2016-07-14 04:28

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Frealign

Hi everybody,

is there any documentation for frealign_calc_variance? It sounds like a helpful tool but I don't fully understand how it works and how to interpret the results.

Cheers
Lukas

niko Thu, 2016-07-14 08:22

frealign_calc_variance is an experimental feature that was built for a collaborator. It is supposed to calculate a given number of reconstructions using randomly selected subsets of the dataset, and estimating the variance of the structure based on these subsets (bootstrap method). This method has been described in Penczek et al. (2006) Estimation of variance in single-particle reconstruction using the bootstrap technique, J Struct Biol 154, 168–183.