Generating initial map

Submitted by papai on Wed, 2016-07-20 05:45

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Frealign

Hi everyone,

Reading the latest article about Frealign in the Methods in Enzymology, I have two questions about the "generating initial map" part:
1. Do you change the high-resolution limit to calculate class membership with the refinement resolution limit?
2. If I understood it well Mode 3 is used in the whole process, do you change the value of DANG during the cycles? If not what value do you use?

Thank you!

Best regards,
Gabor

niko Wed, 2016-07-20 11:18

You can keep the high-resolution limit for classification to a value higher than the current refinement limit. Frealign will then automatically adjust it down to the refinement limit. For DANG, just set it to 0. Frealign will set it internally to the right value.