Using sum_movie to generate aligned stacks?

Submitted by oliverbclarke on
Forums
Unblur & Summovie

Hi,

I've used unblur for whole frame alignment, and I'd like to try polishing in relion (but I only generated the aligned sums initially, not aligned stacks).

I still have the shifts files, so I'd like to use them with sum_movie to generate aligned stacks, to speed up the process and ensure that the overall alignment is identical (which I am not 100% sure is guaranteed if I run unblur again to do so).

Thoughts/suggestions?

Cheers

Oli

jstreetley Sun, 2018-03-04 08:42

Hi Tim (long time, no see!)

I'd also like a copy of summovie that can output frames if possible. The two links in the this thread have already expired. I'd like to use it to dose-weight a tilt series, so I was thinking of giving it an alignment file of 0 0, but obviously a sum makes no sense in that context.

Cheers,

James

anans Tue, 2018-05-22 09:30

Hi Tim,

Could you please post the link to the version of summovie that outputs the dose-weighted stack file? The link doesn't appear to work. This would be great for a quick dose-weighting of tilt series.

Thanks

Andrea