Questions about the filament selection and the saved box files.
In the tutorial (Ab(40) using Frealix) on line (http://grigoriefflab.janelia.org/frealix/abeta_tutorial), it said:
"With "Select" selected on the tools window, begin by picking an endpoint. This endpoint (as shown by the green arrow) should be located along the filament near its starting end, and should not be a crossover.
Following the filament, select the next crossover points in order.
After the last crossover has been selected, pick an endpoint near the end of the filament."
1. This description gives my first set of questions, does this mean the first box and the last box should not be the crossover? Or should I just select the crossovers with boxes.
"When a full filament has been selected, click on Boxes -> Save Box DB in the tool box (or Ctrl+S) to save your box file in the "boxes" directory.
After the file has been saved, click on Boxes -> Clear Boxes in the tool box.
Repeat the previous steps until all good filaments in the films have been picked."
2. Does this mean, I should save a box file for each filament? If I have 10 filaments in each image, I should have 10 box files for each image, which means I will have about 1000 box files since I have about 100 images. I was told the name of box file should match with the mrc file name. Do you have any suggestions about the name of the box files? Also the data size will be extremely large if I have 1000 box files which will bring me more difficulties. Any suggestions in terms of the data size?