from spider alignment file to FREALIGN
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Dear all,
I try to convert my spider alignment file to Frealign parameter file. It always results a sphere in the reconstruction. I suppose something is wrong with my angles, but I couldn't figure out what. Here is the corresponding part of the script I'm using (input for the angles is the doc file from 'ap ref' in spider):
PSI=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $8}'` THETA=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $4}'` PHI=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $5}'` Sx=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $9}'` Sy=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $10}'` FILM=`cat mic_num.plt | awk NR==$cou | awk '{print $1}'` DF1=`cat defocus.plt | awk NR==$cou | awk '{print $1}'` DF2=`cat defocus.plt | awk NR==$cou | awk '{print $2}'` AngAst=`cat defocus.plt | awk NR==$cou | awk '{print $3}'` echo "" | awk '{printf "%7d%8.2f%8.2f%8.2f%8.2f%8.2f%7s%s%6d%9.1f%9.1f%8.2f%7.2f\n",'${cou}',-'${PSI}',-'${THETA}',-'${PHI}','${Sx}','${Sy}','${MAG}',".",'${FILM}','${DF1}','${DF2}','${AngAst}',0.0 }' >> parameters.par
I would appreciate any help!
Thanks in advance,
Gabor
Hi Papai, I can't comment on
Hi Papai,
I can't comment on your angles, but I had a similar problem when trying to refine a C1 object when the symmetry in frealign was set to I2. Is your symmetry card correct?
Axel
Hi Axel, I have C2 symmetry,
In reply to Hi Papai, I can't comment on by Axel
Hi Axel,
I have C2 symmetry, I set the symmetry to "C2".
Gabor
Perhaps this post will be of
Perhaps this post will be of some help?
http://emlab.rose2.brandeis.edu/node/753
Thanks. I saw this post, I've
In reply to Perhaps this post will be of by Axel
Thanks.
I saw this post, I've got the script from there. Unfortunately I made something wrong or missed something, but can't figure out what.