from spider alignment file to FREALIGN

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Dear all,

I try to convert my spider alignment file to Frealign parameter file. It always results a sphere in the reconstruction. I suppose something is wrong with my angles, but I couldn't figure out what. Here is the corresponding part of the script I'm using (input for the angles is the doc file from 'ap ref' in spider):

PSI=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $8}'`
THETA=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $4}'`
PHI=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $5}'`
Sx=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $9}'`
Sy=`cat alignment_1_00001.spi | awk NR==$a | awk '{print $10}'`
FILM=`cat mic_num.plt | awk NR==$cou | awk '{print $1}'`
DF1=`cat defocus.plt | awk NR==$cou | awk '{print $1}'`
DF2=`cat defocus.plt | awk NR==$cou | awk '{print $2}'`
AngAst=`cat defocus.plt | awk NR==$cou | awk '{print $3}'`
	
echo "" | awk '{printf "%7d%8.2f%8.2f%8.2f%8.2f%8.2f%7s%s%6d%9.1f%9.1f%8.2f%7.2f\n",'${cou}',-'${PSI}',-'${THETA}',-'${PHI}','${Sx}','${Sy}','${MAG}',".",'${FILM}','${DF1}','${DF2}','${AngAst}',0.0 }' >> parameters.par

I would appreciate any help!

Thanks in advance,

Gabor

Hi Papai,

I can't comment on your angles, but I had a similar problem when trying to refine a C1 object when the symmetry in frealign was set to I2. Is your symmetry card correct?

Axel