sparx to frealign parameter file

Submitted by giuliazanetti on Mon, 2013-06-24 06:43

Forums

Frealign

Hi, I am trying to convert parameters from sparx alignments to frealign, in order to use the tiltdiff software for tilt validation. I am in particular at loss with the Euler angles conversion.
I have found various solutions on this and other websites, all of them different, and all of them not working in my hands...

Hopefully someone can help me out of this very frustrating task I got into?

Thanks
Giulia

niko Thu, 2013-06-27 16:53

I think your best bet is to use the conversion routines provided by EMAN2. If you would like to check that the conversion was successful you may want to calculate matching projections in Frealign and check that they are matching your particles. You can also try using Frealign to re-determine the orientations from scratch for the tilt test.