reconstruct references separately?
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I am trying multi-reference refinement, but am getting crashes during reconstruction, apparently because the two reconstructions running simultaneously demand more memory than is present. Is there a way to reconstruct the r1 volume and the r2 volume separately, at different times?
Alternately, I guess I could do two runs and specify half of the particles in calc_reconstructions for each, then recombine the two halves for each.
Yes, if you want to run the
Yes, if you want to run the reconstructions at different times you will have to do it manually. You could also adapt your script so it only calculated one reconstruction at a time. In the bin directory, inside script mult_reconstruct.com, find the following lines:
if ( $sym == "H" || $sym == "HP" ) then
${bin_dir}/mult_hreconstruct_n.com $firstn $lastn $start $nc &
else
${bin_dir}/mult_reconstruct_n.com $firstn $lastn $start $nc &
endif
and remove the "&" symbol at the end of the two long lines.
great, thank you!
In reply to Yes, if you want to run the by niko
great, thank you!