Frealign with phase plate data? | The Grigorieff Lab

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Frealign

Hi everyone,

I was wondering if there were any plans to implement frealign refinement with phase plate data.

Best,

Axel

This may well be implemented

niko Sun, 2016-03-20 19:41

This may well be implemented in a future version, but probably not for a few months.

I may be interested in

Axel Sun, 2016-03-20 19:59

I may be interested in testing out a beta version when this becomes available.

Hi all, I'm in the same

lizkellogg Mon, 2017-05-29 18:49

Hi all,

I'm in the same position as Axel, I would like to try out frealign with support for VPP datasets. Any ETA on when this version will be released?
Thanks

Liz

We plan to release the new

niko Tue, 2017-05-30 01:42

We plan to release the new version in one to two months from today.

frealign for VPP dataset

Jamon Wed, 2017-04-05 04:35

Dear Niko,
I am wondering if there is new version of frealign that can handel VPP data now. I have a few VPP datasets and keen to try out frealign for 3D refinement.

Thanks,
Jian
CryoEM, CBIS, NUS

A version of Frealign that

niko Wed, 2017-04-05 05:15

A version of Frealign that implements phase shifts is currently being tested and will soon be released.

Niko, Thanks for the

Jamon Mon, 2017-04-10 04:35

Niko, Thanks for the information.
I am also wondering if it's possible just run current Frealign on VPP dataset, only using defocus value but without phase shift? Anything bad would happen?

Regards,
Jian

Just give it a try.

niko Mon, 2017-04-10 04:38

Just give it a try. Presumably, the CTF will not be correct and you will loose resolution.