Error: Part_fsc cannot estimate resolution well



We are trying to refine structure with FrealignX, but the Part_fsc value never went down below 0.143 to give a resolution estimation at global search. This has occurred for a few datasets in the lab. We are wondering what the reason could be?

Thank you so much for your time!

This problem is sometimes seen when particles are close together and there are images in the particle stack with more than one particle inside the box. It is then possible that the same particle ends up being used more than once in the reconstruction. Try to limit the search range so it can only align particles in the center of the boxes.