Creating large image stack with em2em

Submitted by Unknown on Wed, 2009-12-09 17:43

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Frealign

I have a problem to convert the imagic stack file of boxed particle into mrc stack file using em2em due to the large file size (~8 GB). Is it possible to have the normalized imagic stack image file as the input image file for running frealign? Is so, how about the format of initial model and the parameter file?

Also, do you have or know any program other than em2em that can convert large data set from imagic stack to mrc stack?

niko Wed, 2009-12-09 17:44

em2em should be able to write large MRC files. Are you running on a 32 bit machine?

The new Frealign version (the one on our web page) can read IMAGIC file format. Just change the M in card 1 to I. All the other image data (including initial model or reference) will also have to be supplied in IMAGIC format. The particle parameters are the same as with MRC or Spider format.