Relion did not find any of the expected EPS files


I've been trying to use CTFFIND-4.1 to do CTF estimation in relion-3.0, but I have been unsuccessful due to relion not finding the expected EPS files. Each time, none are produced and the .star file made is empty.

I'm fairly certain I have compiled and installed CTFFIND 4 correctly on my OS but seem to be missing something. I have used CTFFIND3 before in relion-2.0 on another machine. I'm wondering if there are extra dependencies (I have just recently built my OS with centOS 8) or if there may be a certain step I may be missing?

Thanks for any advice you can provide!


You should check whether your compiled CTFFIND4 works. Just open a terminal, and try to run the ctffind binary you compiled. If that works (i.e. gives a prompt asking for an input file), you know you built it correctly.

I don't know what EPS files are, so I'm not sure what their not being found implies.


In reply to by Alexis

Thanks for the suggestion. I tried to run ctffind in the terminal, but keep receiving the error:

ctffind: error while loading shared libraries: cannot open shared object file: No such file or directory

However, I installed WxWidgets 3.1 and still received this error. Have you encountered something like this before?

Thanks again,

In reply to by mshred

Hi Madeline,

Before compiling cisTEM, are you passing an argument to config to tell it where to find your wx? I.e. something like --with-wx-config=/path/to/wxconfig