Relion did not find any of the expected EPS files

Submitted by mshred on Thu, 2019-10-03 15:32

Forums

ctffind & ctftilt

Hi,

I've been trying to use CTFFIND-4.1 to do CTF estimation in relion-3.0, but I have been unsuccessful due to relion not finding the expected EPS files. Each time, none are produced and the .star file made is empty.

I'm fairly certain I have compiled and installed CTFFIND 4 correctly on my OS but seem to be missing something. I have used CTFFIND3 before in relion-2.0 on another machine. I'm wondering if there are extra dependencies (I have just recently built my OS with centOS 8) or if there may be a certain step I may be missing?

Thanks for any advice you can provide!

Alexis Thu, 2019-10-03 15:52

Hi,

You should check whether your compiled CTFFIND4 works. Just open a terminal, and try to run the ctffind binary you compiled. If that works (i.e. gives a prompt asking for an input file), you know you built it correctly.

I don't know what EPS files are, so I'm not sure what their not being found implies.

HTH
Alexis

mshred Fri, 2019-10-04 16:01

Thanks for the suggestion. I tried to run ctffind in the terminal, but keep receiving the error:

ctffind: error while loading shared libraries: libwx_baseu-3.1.so.2: cannot open shared object file: No such file or directory

However, I installed WxWidgets 3.1 and still received this error. Have you encountered something like this before?

Thanks again,
Madeline

himesb Sat, 2019-10-05 17:24

Hi Madeline,

Before compiling cisTEM, are you passing an argument to config to tell it where to find your wx? I.e. something like --with-wx-config=/path/to/wxconfig