Visualized cryo-EM experiment
Just out, this video publication in the Journal of Visualized Experiments by the lab of Peijun Zhang, which takes us through the reconstruction of a HIV-1 capsid tube assembly from freeze plunging to final reconstruction. Though the actual method may only be of interest to those working on helical projects, this may well be the first video publication on cryo-EM you watch!
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
Xin Meng, Gongpu Zhao, Peijun Zhang
Department of Structural Biology, University of Pittsburgh School of Medicine
Grant Jensen has had a
Grant Jensen has had a tomography one on there for over a year! Looks like it's got a higher production budget too! :)
http://www.jove.com/details.php?id=1943