SerialEM Tips - Stage Position Z

Moving stage Z is perhaps more challenging than X,Y. But it is a very reasonable request to ask specimen to move to Eucentric Height at each location you want to record the final image. With SerialEM, it can be done relatively easy. There is still a room to improve the accuracy, taking into account the facts like normalize objective lenses etc.. Here I am trying to explain how it is done.

http://www.bio.brandeis.edu/KeckWeb/emdoc/en_US.ISO8859-1/articles/Seri…