SerialEM Tips - stage position X, Y

We had discussion before using hole template for stage positioning. It works well in most cases. However, there are situations which this might not work so well. For example, when there is no regular hole on the grid, or we do not want every hole. In some cases, we can see the sample position in hole like with filament sample which may not be quite in the center of the hole. In negative stain case, there is no hole.

In general, it would be very nice if we have a way to go to each target position regardless it is a hole or not. Exactly for this purpose, I wrote up a little SerialEM HowTo to show how we can do this. One of the advantages of this way is that it takes care of stage drifting. For the points picked a few hours earlier, this can get to these point with no trouble.

You can find it at

http://www.bio.brandeis.edu/KeckWeb/emdoc/en_US.ISO8859-1/articles/Seri…
or
http://metz.rose2.brandeis.edu/emdoc/articles/SerialEM-howto:positionin…