Dynamic cytoplasmic fluidity during morphogenesis in a human fungal pathogen
Publication Type
Journal Article
Year of Publication
2024
Refereed Designation
Not refereed
Journal
bioRxiv
Volume
2024.11.16.623909
Date Published
11/2024
Abstract
The molecular crowding of the cytoplasm impacts a range of cellular processes. Using a fluorescent microrheological probe (GEMs), we observed a striking decrease in molecular crowding during the yeast to filamentous growth transition in the human fungal pathogen Candida albicans. This decrease in crowding is due to a decrease in ribosome concentration that results in part from an inhibition of ribosome biogenesis, combined with an increase in cytoplasmic volume; leading to a dilution of the major cytoplasmic crowder. Moreover, our results suggest that inhibition of ribosome biogenesis is a trigger for C. albicans morphogenesis.