CTFFIND4 and cs correction

Submitted by rogelio on Tue, 2015-05-26 16:15

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ctffind & ctftilt

Hi there!

I am tryiing to calculate the defocus on a set of images collected on a microscope with a Cs corrector, can CTFFIND4 handle a Cs=0.0?

I am getting the following error

ctffind4 --old-school-input
15may21z_May15_19.13.54_3145.mrc
15may21z_May15_19.13.54_3145-pow.mrc
0.0,300.0,0.07,59000.0,6.136,1
512,30.0,4.0,5000.0,35000.0,500.0,100.0
forrtl: error (73): floating divide by zero
Image PC Routine Line Source
ctffind4 00000000016B81B9 Unknown Unknown Unknown
ctffind4 00000000016B6B30 Unknown Unknown Unknown
ctffind4 00000000015D85D2 Unknown Unknown Unknown
ctffind4 0000000001578928 Unknown Unknown Unknown
ctffind4 000000000157F7E1 Unknown Unknown Unknown
ctffind4 0000000001563EB0 Unknown Unknown Unknown
ctffind4 0000000001759699 Unknown Unknown Unknown
ctffind4 0000000001753E6B Unknown Unknown Unknown
ctffind4 000000000174B8D3 MAIN__ 533 ctffind.f90
ctffind4 0000000000400566 Unknown Unknown Unknown
ctffind4 00000000016C1DFB Unknown Unknown Unknown
ctffind4 0000000000400429 Unknown Unknown Unknown

Thanks!

Rogelio

Alexis Tue, 2015-05-26 16:18

Hi Rogelio,

Sorry about that - it shouldn't crash on you. Which version of ctffind4 is this?

In any case, just try Cs=0.001mm and it should work.

Cheers,
Alexis