FindDQE on Gatan K2 with energy filter
Hi,
I've been using FindDQE to characterize our cameras and monitor them over time for any arising problems. Recently we installed a new microscope with a Gatan K2 behind an energy filter. The results from FindDQE are significantly worse than on our other K2. Talking to Gatan, they said it was because the pointer is too far from the final image, and that the mask they have right in front of the camera would be the proper solution. Unfortunately FindDQE does not like this mask image. I think this is because the dark areas cross all of the borders of the screen. Is it possible to modify the software so it will like this sort of image?
Attached are the mask image, and the MTF plot comparing K2 on a scope without an energy filter (Krios1) with one with an energy filter (Krios2), both calculated using the pointer.
Thanks,
Bill
Gatan is correct that FindDQE
Gatan is correct that FindDQE fails when the pointer image is blurry. The software could be modified to deal with different geometries but there are no plans to do so at the moment.
FindDQE with K3 behind Bioquantum
In reply to Gatan is correct that FindDQE by niko
Gatan is correct that FindDQE fails when the pointer image is blurry. The software could be modified to deal with different geometries but there are no plans to do so at the moment.
We are working on a new
In reply to FindDQE with K3 behind Bioquantum by bobg
We are working on a new version of FindDQE that should be usable with an edge generated by the Bioquantum mask. The new version might be done in about a month from now. Please check back here.
Bioquantum mask
In reply to We are working on a new by niko
Hi,
Just wondering if the updated version of FindDQE will be released soon that works with the edge generated by the Bioquantum mask?
Thank you
Andrea
We are still working on an…
In reply to Bioquantum mask by anans
We are still working on an updated version of FindDQE. However, apart from the geometry of the Bioquantum mask, we also have to solve more general problems that have to do with the non-linear behavior of counting detectors. Please see this post for more information:
https://grigoriefflab.umassmed.edu/forum/software/finddqe/falcon3_counting_mode