Huge MTF error for sample data

Submitted by nihy on Sat, 2018-12-01 21:22

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FindDQE

Hi, I am a new user of FindDQE. When I was running sample data, I found a huge difference of MTF compared to provided result. When I was testing DE12, it even gave a DQE near 3.0, so I would like to ask what can be the source of this error? The parameter I input was the same to the .com file provided.

Thanks in advance.

Haoyang

niko Sat, 2018-12-01 21:24

The exposure you entered could be incorrect. How did you calibrate the exposure?

nihy Sat, 2018-12-01 22:09

As in your script, I chose total dose(2) and input 52.8 for total electron dose/pixel.

The following is a copy of my input in bash. What puzzles me is in the reference paper, it gives that DE12 data collection with 13 eps and 25 frames per second, so I wonders how 52.8 total dose/pixel comes from.

You can input either the counts/electron (gain conversion factor) or the total electron dose/pixel.
Enter 1 to input counts/electron, 2 for total dose/pixel
2
2

Enter total electron dose/pixel
52.8
52.7999992

Enter counts multiplier (usually 1).
1
1.00000000

Enter number of images
(usually 1; 2 if also giving flat field image)
1
1

Input image with pointer silhouette
DE12-200kv-50e-13eps.mrc
DE12-200kv-50e-13eps.mrc