unblur.com & summovie.com

Submitted by Ludo on Fri, 2015-06-05 12:19

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Unblur & Summovie

Hi,

I wanted to give a go at unblur and summovie.
I'm unsure how to launch them on a folder full of mrc stacks?
I guess I should create a .com file very much like rmeasure is using but I'm not sure what it should contain.
Could you give me an example of how to do that?

Thank you,
Ludo

timgrant Fri, 2015-06-05 17:05

Hi Ludo,

You can write a script to run it, just like the .com file in rmeasure.

The number and order of arguments differs depending on which options you select, so your best bet would be to run it interactively using the parameters you want - then use that order to create the script.

For example the following will align "input_movie.mrc" which contains 100 frames, create an aligned sum called "aligned_sum.mrc", and a text file containing the shifts ("movie_shifts.txt") - a pixel size of 1A, with dose filtering set to YES, and thus an exposure per frame of 1.4e/A2 at 300kv. Expert options is set to NO. : -

#!/bin/csh
#
./unblur 

Hope that helps!


Tim

Ludo Mon, 2015-06-08 05:05

Thanks.
That's what I needed.

I edited my unblur.com as this:

#!/bin/csh
#
./unblur 

but I can't start a loop on this.

foreach i (*.mrc); ./unblur.com; end

it doesn't work.

Sorry for being bad at this. I never had to run an interactive script like this.

How do I tell to run on all the mrc in the folder with these parameters.

Must sounds very basic to you ... THANKS!

timgrant Mon, 2015-06-08 12:06

Hi Ludo,

I think you are using bash format in your script and csh doesn't like it. You're probably better off sticking with bash - in which case the following script should achieve what you want in combination with *.mrc (assuming the only mrc files in the directory are movie frame stakcks):-

!/bin/bash
#
for i in "$@"
do
./unblur 

Thanks,


Tim

Ludo Tue, 2015-06-09 05:20

Hi,
Me again,
If I do that script, nothing happens.
If I replace "$@" by *.mrc then all the mrc files are read with the options but only the last one is aligned.

forrtl: severe (24): end-of-file during read, unit 5, file /proc/21416/fd/0

I get this forrtl error except for the last file of the folder that is being aligned fine.
a quick google tells me it's run out of data ...?

what did you mean by "in combination with *.mrc"?
Thanks.

Ludo Tue, 2015-06-09 11:38

seems to be working.
Thanks very much for the help!

I have another question.
After running unblur, I can use the shifts file to run summovie.
Summovie also generates and average. I couldn't find a reference to summovie in the elife paper.
Did I miss it?

Thanks.

timgrant Tue, 2015-06-09 11:46

Hi Ludo,

Yes summovie essentially does the same thing as unblur, just without the alignment. It's included purely so you can try different options for the dose filter or make sums for different ranges of movie frames without re-running the alignment.

Not that it is a very important point - but in both cases the programs generate sums.

BertramDaum Wed, 2017-11-22 08:23

Hi Tim,
this may be a silly question, I'm relatively new to the single particle field. Do I assume correctly that summovie uses the unaligned stacks as input files and then applies the alignment using the shift files generated by a previous unblur run?

Can I also run summovie on aligned stacks and if not, do you have a script at hand that can do that?

Many thanks,
B

timgrant Wed, 2017-11-22 18:55

Yes, this is correct.

The way summovie is setup, you need to provide a shift file, and these shifts are applied before summing. If you wanted to use an aligned stack, then you could always provide a shift file that was all 0.0. This would apply no shifts, and just sum the movie.

Cheers,

Tim