Fatal error (ctffind): Input stacks are only supported when using the modern UI

I am getting this message in the .log file using ctffin4:

"Fatal error (ctffind): Input stacks are only supported when using the modern UI"

What does it mean?

The file that is being processed is attached

Hi,

Your MRC file is slightly weird, and CTFFIND is not able to read it properly. Out of interest, what detector / acquisition program is this from?

IMOD and EMAN2 are both able to read it, and thus can convert it to a more normal MRC file.

You can convert with IMOD by running newstack --input GS_RAw_SPA063.mrc --output GS_RAw_SPA063_rewrite.mrc. You can convert with EMAN2 by running e2proc2d.py GS_RAw_SPA063.mrc GS_RAw_SPA063_rewrite.mrc

CTFFIND should then run on the rewrite file.

Thanks,

Tim

In reply to by timgrant

Thank you Tim.

I did as you recommended and everything works perfectly now;)

The files were initially saved as .tif by EMMENU4 software and exported as .mrc. DE20 camera was used.
Now I did tif to mrc conversion by IMOD and have no issues anymore. Thank you for assisting me in this ..