Hi, I am trying to correct micrographs collected from K2 Summit using mag_distortion serial programs. But I have faced some problems about magdistortion.
1. Can the micrographs (not crystalline areas, such as virus or proteins) be used for distortion estimation? I have tested some images from different types of grids with various samples. It seems that the major scale and minor scale are the same in the same batch of micrographs. But the distortion angles are quite different in the same batch.
2. If the micrographs collected can be used for distortion estimation. What's the optimal resolution shell for estimation process?