FREALIGN: high-resolution refinement of single particle structures
Publication Type
Journal Article
Year of Publication
2007
Refereed Designation
Refereed
Journal
J Struct Biol
Volume
157
Pagination
117-125
Citation Key
99
Number
1
Keywords
Algorithms, Electron, Image Enhancement, Imaging, Microscopy, Particle Size, Software, Software Design, Three-Dimensional
Abstract
The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page.
