Ab initio resolution measurement for single particle structures
Publication Type
Journal Article
Year of Publication
2007
Refereed Designation
Refereed
Journal
J Struct Biol
Volume
157
Pagination
201-10
Date Published
Jan
ISSN
1047-8477 (Print)
Accession Number
17029845
Citation Key
55
Number
1
Keywords
Escherichia coli, Fourier Analysis, Image Enhancement/*methods, "Imaging, Three-Dimensional/*methods", Macromolecular Substances/chemistry, "Microscopy, Electron/*methods", "Models, Molecular", Ribosomes/chemistry, Ryanodine Receptor Calcium Release Chann
Abstract
A computational method is described that allows the measurement of the signal-to-noise ratio and resolution of a three-dimensional structure obtained by single particle electron microscopy and reconstruction. The method does not rely on the availability of the original image data or the calculation of several structures from different parts of the data that are needed for the commonly used Fourier Shell Correlation criterion. Instead, the correlation between neighboring Fourier pixels is calculated and used to distinguish signal from noise. The new method has been conveniently implemented in a computer program called RMEASURE and is available to the microscopy community.
