Noise power

Submitted by nfischer on Tue, 2015-07-21 02:48

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Unblur & Summovie

Dear all,

I am wondering what the "restore noise power after filtering" function actually means, as I could not find a description in the eLife paper. By default this option is set to yes in unblur and sum_movie. What is it good for and in which situations should it be used and in which situations rahter not?

Thanks again for any help and best resgards
Niels

timgrant Tue, 2015-07-21 11:25

Hi Niels,

This is essentially the denominator in equation 9 in the paper. Without it, the resulting image is quite low pass filtered.

You probably always want to say YES here, but we sometimes make a version with NO for particle picking as it seems to be quite a nice low-pass filter in some cases.

Tim