Quantitative characterization of electron detectors for transmission electron microscopy

Publication Type

Journal Article

Year of Publication

2013

Refereed Designation

Refereed

Journal

J Struct Biol

Volume

184

Pagination

385-393

Date Published

2013 Nov 1

ISSN

1095-8657

Citation Key

4236

Abstract

A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of their modulation transfer function (MTF) and detective quantum efficiency (DQE). However, the performance of these new detectors needs to be carefully monitored in order to optimize imaging conditions and check for degradation over time. We have developed an easy-to-use software tool, FindDQE, to measure MTF and DQE of electron detectors using images of a microscope's built-in beam stop. Using this software, we have determined the DQE curves of four direct electron detectors currently available: the Gatan K2 Summit, the FEI Falcon I and II, and the Direct Electron DE-12, under a variety of total dose and dose rate conditions. We have additionally measured the curves for the Gatan US4000 and TVIPS F416 scintillator-based cameras. We compare the results from our new method with published curves.

Alternate Journal

J. Struct. Biol.