Ito channels are octomeric complexes with four subunits of each Kv4.2 and K+ channel-interacting protein 2
Year of Publication
J Biol Chem
*Ions, "*Potassium Channels, Voltage-Gated", Animals, Calcium-Binding Proteins/*chemistry, "Centrifugation, Density Gradient", Charybdotoxin/pharmacology, COS Cells, "Dose-Response Relationship, Drug", "Electrophoresis, Polyacrylamide Gel", Electrophysiol
Mammalian voltage-gated K+ channels are assemblies of pore-forming alpha-subunits and modulating beta-subunits. To operate correctly, Kv4 alpha-subunits in the heart and central nervous system require recently identified beta-subunits of the neuronal calcium sensing protein family called K+ channel-interacting proteins (KChIPs). Here, Kv4.2.KChIP2 channels are purified, integrity of isolated complexes confirmed, molar ratio of the subunits determined, and subunit valence established. A complex has 4 subunits of each type, a stoichiometry expected for other channels employing neuronal calcium sensing beta-subunits.