Reference frame for average | The Grigorieff Lab

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Hi Tim-

Quick question: when unblur outputs an average image, which frame does it use as the reference for the alignment? I.e. is it the first frame, middle frame, etc.?

Thanks,
-Greg Alushin

Hi Greg, The central frame

timgrant Wed, 2015-07-01 13:23

Hi Greg,

The central frame should always have shift 0, so the output shifts are relative to this.

Cheers,

Tim

If it is an even number, is

galushin Wed, 2015-07-01 13:32

If it is an even number, is this frame N/2 + 1?

Yes for even it is N/2 + 1,

timgrant Wed, 2015-07-01 13:40

Yes for even it is N/2 + 1, for odd it is (N + 1) / 2

You can double check this by looking at the output shifts file, the frame with a 0 shift is the middle frame.

Tim

Great thanks! -g

galushin Wed, 2015-07-01 13:46

Great thanks!
-g