I have been trying to use the dose weighting of Summovie to improve my final reconstruction.
Initially I used sums without dosage correction, thereby I reach a final resolution of 3.3A. I then wanted to try the dosage correction.
For this I created new sums of my previously aligned movies. I used the par file of my 3.3A Volume and the particles from the new dose filtered sums, I end up with 4.24A, which improves to 3.95A after another 4 rounds of refinement. My movies consist of 10 frames with a dose of 3.1 e/A²/frame and a pre exposure of 4.6 e/A².
Do you have some input on the unexpected resolution difference?
Is there a dose range in which Summovie works optimally?