Recently I could not apply gain reference by using Mag_distortion_correction. What happened is, after applying gain reference, unblur could not align frames correctly and the result image looked terrible. I guess this is due to the update affecting tif2mrc command. So I applied gain reference by using "clip mult -n 16 img.mrc gain.mrc gain_applied.mrc, then used unblur. This worked well. But the problem is... the values in the shift file are slightly different from what I got before with mag_distortion_correction. So I wonder how different mag_distortion_correction & clip command are in applying gain reference. For me, mag_distortion_correction seems working better. So, I wonder whether this issue can be fixed..
Thanks a lot,