Strange CTF prediction - CTFfind

Submitted by taianah on Fri, 2017-02-17 07:54

Forums

ctffind & ctftilt

Dear Nikos,

I encountered this kind of prediction throughout a k2 dataset that was pre-treated with unblur for alignment of frames (no Dosage filtering) i got puzzeld as I have not seen this kind of thing before. Would
you have any insights about it and how to correct it?

Thanks in advance for your attention and help,

Taiana

niko Fri, 2017-02-17 08:57

The patterns you see there are due to aliasing. You can avoid this by making the box size bigger, or by using a lower defocus.