Re: Defocus estimation on images with hardly any thon rings

Submitted by chunfuxu on Tue, 2013-06-11 12:48

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ctffind & ctftilt

Hi Axel,

Please see the attached image. I just realized that the cryo-scope we collected data on is a phase plate system. I don't know if this could have an effect.

Thank you!

-Chunfu

Axel Tue, 2013-06-11 12:55

I imagine it would have an effect. Why are you defocusing if you have a phase plate?

Axel